Fast chemiluminescent measurement of RNA polymerase activity based on photon counting technology

¹Abteilung Biochemische Kinetik, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, FRG.

|November 1, 1993

Abstract:

A fast and simple assay for T7 RNA polymerase based upon chemiluminescent detection of the synthesized, digoxigenin-labeled RNA on a nylon membrane with anti-digoxigenin coupled alkaline phosphatase and CSPD as substrate is described. Activity of RNA polymerase is determined with high sensitivity by quantifying the emitted light of the microplate-formatted dot-blot membrane with a photon counting microplate luminometer and a specially designed filter adapter. The described method is one example for the application of this new adapter to measure luminescent membrane filters.

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Abstract:

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