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Generation of Human CD40-activated B cells
13:27

Generation of Human CD40-activated B cells

Published on: October 17, 2009

Ligation of CD40 induces sterile transcripts of multiple Ig H chain isotypes in human B cells

M D Jumper1, J B Splawski, P E Lipsky

  • 1Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas 75235.

Journal of Immunology (Baltimore, Md. : 1950)
|January 15, 1994
PubMed

Stimulation of human B cells with mAb to CD40 in the presence of IL-4 induces proliferation and differentiation into Ig-secreting cells. To delineate the molecular events leading to Ig secretion after stimulation via the CD40 molecule, the induction of germ-line transcripts of Ig H chain isotypes was analyzed by polymerase chain reaction. The results document that costimulation with mAb to CD40 and IL-4 induces sterile transcription of all Ig H chain isotypes. Of importance, stimulation with mAb to CD40 without the addition of IL-4 induced germ-line transcription of most downstream isotypes, suggesting that this signal is sufficient to initiate the first step in switch recombination.

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