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What makes an mRNA anti-sense-itive?
1Max-Planck-Institut für Biochemie, Martinsried, Germany.
Trends in Biochemical Sciences
|November 1, 1993
Summary
Antisense RNA can silence gene expression. While mechanisms are known in prokaryotes, eukaryotic gene silencing by antisense transcripts is poorly understood, though endogenous examples suggest regulatory roles.
Area of Science:
- Molecular Biology
- Gene Regulation
- RNA Biology
Background:
- Antisense RNA is a tool for gene silencing.
- Gene regulation by endogenous antisense transcripts is established in prokaryotes.
- Mechanisms of antisense-mediated gene silencing in eukaryotes are not well understood.
Purpose of the Study:
- To discuss a model explaining antisense-mediated gene silencing mechanisms in eukaryotes.
- To highlight the discovery of endogenous eukaryotic antisense transcripts.
- To explore the regulatory functions of these transcripts.
Main Methods:
- Literature review of antisense RNA applications.
- Analysis of prokaryotic and eukaryotic gene regulation models.
- Discussion of proposed mechanisms for antisense-mediated silencing.
Main Results:
- Antisense gene constructs are routinely introduced into eukaryotes.
- Endogenous eukaryotic antisense transcripts have been discovered.
- These transcripts likely play regulatory roles.
Conclusions:
- A model for antisense-mediated gene silencing is presented.
- Further research is needed to fully elucidate eukaryotic mechanisms.
- Endogenous antisense transcripts are important in gene regulation.