Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endogenous digitalis-like activity in the newborn

S Ghione1, S Balzan, S Decollogne

  • 1CNR Institute of Clinical Physiology, Pisa, Italy.

Journal of Cardiovascular Pharmacology
|January 1, 1993
PubMed
Summary

Researchers identified an endogenous digitalis-like factor in umbilical cord blood. This factor exhibits biological activity similar to digitalis, confirmed by its interaction with antidigoxin antibodies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevated mortality risks associated with late diagnosis of cancer in individuals with psychiatric disorders?

Journal of psychiatric research·2024
Same author

Sex differences in the pharmacokinetics of anticancer drugs: a systematic review.

ESMO open·2024
Same author

Prognostic value of total, free and lipoprotein fraction-bound plasma mitotane levels in advanced adrenocortical carcinoma: a prospective study of the ENDOCAN-COMETE-Cancer network.

Journal of endocrinological investigation·2024
Same author

Association of cabozantinib pharmacokinetics, progression and toxicity in metastatic renal cell carcinoma patients: results from a pharmacokinetics/pharmacodynamics study.

ESMO open·2021
Same author

Effects of azacitidine in 93 patients with <i>IDH1/2</i> mutated acute myeloid leukemia/myelodysplastic syndromes: a French retrospective multicenter study.

Leukemia & lymphoma·2020
Same author

Antineoplastic busulfan encapsulated in a metal organic framework nanocarrier: first in vivo results.

Journal of materials chemistry. B·2020

Area of Science:

  • Endocrinology and Metabolism
  • Cardiovascular Physiology
  • Biochemistry

Background:

  • Elevated endogenous digoxin-like immunoreactivity is observed in premature infants, term pregnancy, amniotic fluid, and human milk.
  • These factors suggest the presence of endogenous digitalis-like factors (EDLFs) with potential biological activity.
  • Umbilical cord blood is identified as a readily accessible source for isolating these endogenous compounds.

Purpose of the Study:

  • To partially purify and characterize an endogenous digitalis-like factor from human umbilical cord blood.
  • To provide direct evidence linking digoxin-like immunoreactivity with biological digitalis-like activity.
  • To investigate the selectivity and nature of this endogenous compound.

Main Methods:

  • Partial purification of the endogenous factor from umbilical cord blood.

Related Experiment Videos

  • Assay of inhibitory activity on 86Rubidium (86Rb) uptake.
  • Neutralization of activity using antidigoxin antibodies (Fab fragments).
  • Immunoaffinity chromatography and high-performance liquid chromatography (HPLC) for purification and separation.
  • Testing for selectivity (tissue, isoenzyme) and identification against known steroids.

    • Main Results:

    • The inhibitory activity of the purified factor on 86Rb uptake was neutralized by antidigoxin antibodies.
    • This neutralization provided the first direct evidence of an association between digoxin-like immunoreactivity and biological digitalis-like activity.
    • Preliminary findings indicate tissue and isoenzyme selectivity, and the compound is not a known steroid.

    Conclusions:

    • An endogenous digitalis-like factor with biological activity has been successfully isolated and partially purified from umbilical cord blood.
    • Antidigoxin antibodies can be used to confirm the digitalis-like nature of this factor and as a tool for purification.
    • The endogenous compound exhibits selectivity, suggesting a specific physiological role yet to be fully elucidated.