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Related Experiment Videos

Digitalis-like factors from human urine

A Goto1, K Yamada, N Yagi

  • 1Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

Journal of Cardiovascular Pharmacology
|January 1, 1993
PubMed
Summary

Researchers identified two potential endogenous digitalis-like factors in human urine. These compounds, ODC-1 and ODC-2, show similarities to ouabain and digoxin, suggesting mammalian synthesis of cardenolide-like substances.

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Area of Science:

  • Biochemistry
  • Pharmacology
  • Human Physiology

Background:

  • The existence of endogenous digitalis-like factors (EDLFs) in mammals is hypothesized.
  • These factors may play a role in regulating cardiovascular function.
  • Human urine is a potential source for isolating such endogenous compounds.

Purpose of the Study:

  • To isolate and characterize potential endogenous digitalis-like factors from human urine.
  • To investigate the properties and similarities of these isolated compounds to known cardiac glycosides.

Main Methods:

  • Isolation of compounds from human urine based on inhibition of [3H]ouabain binding to erythrocytes.
  • Characterization using biological assays, physicochemical analysis, and chromatographic techniques (HPLC, TLC).

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  • Structural elucidation using Fast Atom Bombardment Mass Spectrometry (FAB-MS) and Proton Nuclear Magnetic Resonance (1H NMR) spectroscopy.

    • Main Results:

    • Two distinct ouabain-displacing compounds (ODC-1 and ODC-2) were isolated.
    • ODC-1 exhibited properties similar to ouabain and its action was neutralized by anti-ouabain IgG.
    • ODC-2 demonstrated identical chromatographic behavior to digoxin, with spectral data suggesting it may be indistinguishable from digoxin.

    Conclusions:

    • Human urine contains at least two endogenous substances that mimic the effects of cardiac glycosides.
    • ODC-1 and ODC-2 represent potential candidates for endogenous digitalis-like factors.
    • The findings support the hypothesis that cardenolide-like substances are synthesized within the mammalian body.