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Spatially restricted expression of set mRNA in developing rat kidney
E G Kim1, M E Choi, B J Ballermann
1Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
The American Journal of Physiology
|January 1, 1994
Summary
The Set gene, implicated in leukemia, is expressed in developing rat kidneys. Both alpha and beta Set variants are found, with higher expression in neonatal kidneys, particularly in developing nephron structures.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- Somatic translocation involving the Set gene fused to an oncogene is linked to acute nonlymphocytic leukemia.
- The role of the Set gene in normal physiological processes remains largely uncharacterized.
Purpose of the Study:
- To clone and characterize full-length cDNAs homologous to human Set from rat neonatal kidney.
- To investigate the expression pattern of Set mRNA during rat kidney development.
Main Methods:
- Cloning of full-length cDNAs homologous to human Set.
- Ribonuclease protection assay to detect Set mRNA variants (alpha and beta).
- Northern blot analysis to examine Set mRNA transcript sizes.
- In situ hybridization to localize Set mRNA expression in neonatal rat kidneys.
Main Results:
- Two Set cDNA variants (alpha and beta) with distinct open reading frames and predicted intracellular protein products were identified.
- Both Set variants were expressed in the kidney, with mRNA levels four- to eightfold higher in neonatal compared to adult kidneys.
- Set mRNA expression was predominantly localized to the cortical region of developing nephron structures in neonatal kidneys.
Conclusions:
- The Set gene is expressed in the developing rat kidney, suggesting a potential role in kidney morphogenesis.
- The developmental regulation of Set expression points to its involvement in specific kidney developmental stages.
- Understanding Set expression in kidney development may offer insights into its role in leukemogenesis.