The clastogenic and mutagenic effects of ascorbigen and 1'-methylascorbigen

Ascorbigen, which occurs naturally in the human diet, and a synthetic analogue (1'-methylascorbigen), were assayed for cytotoxic and clastogenic activities in a SV40-transformed Indian Muntjac cell line (SVM), and for mutagenic activity in the Ames test using Salmonella typhimurium strains TA98 and TA100. Ascorbigen had no effect upon the clonal survival of SVM at concentrations below 0.21 mg/ml and did not induce either chromosome aberrations or sister-chromatid exchanges (SCEs) at any concentration tested up to the maximum compatible with the assay conditions; nor did it induce mutations in either Salmonella strain. In contrast, 1'-methylascorbigen was an order of magnitude more cytotoxic, demonstrating a Dq of 0.03 mg/ml, and whilst it too was not found to induce chromosome aberrations it did induce SCEs in SVM (although only at highly cytotoxic doses) and mutations in the Ames test.