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Artificial protein vaccines with predetermined tertiary structure: application to anti-HIV-1 vaccine design
A M Eroshkin1, P A Zhilkin, V V Shamin
1Research Institute of Molecular Biology, NPO VECTOR, Koltsovo, Novosibirsk Region, Russia.
Abstract:
A successful approach to the development of a safe and effective synthetic vaccine requires that different B and T cell epitopes of the infectious agent be included in the vaccine construction. In this paper we suggest a new approach to vaccine design in the form of an artificial protein with a predetermined tertiary structure (PTS vaccines). Based on B and T cell epitope properties, we substantiate the possible use for vaccine construction of one well-known protein spatial motif--the four-alpha-helix bundle. Antigenic determinants of cellular immunity (amphipathic alpha-helices) and humoral immunity (flexible hydrophilic loop regions) are used as blocks for vaccine design. General principles of PTS vaccine construction have been applied to anti-HIV-1 vaccine design.