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Neutrophil adhesion to endothelial cells
O Abbassi1, T K Kishimoto, L V McIntire
1Biomedical Engineering Laboratory, Rice University, Houston, Texas.
Blood Cells
|January 1, 1993
Summary
Neutrophil rolling during inflammation involves E-selectin (ELAM-1) adhesion molecules. Anti-E-selectin antibodies significantly block this initial neutrophil rolling on transfected cells.
Area of Science:
- Immunology
- Cell Biology
- Inflammation Research
Background:
- Neutrophil emigration to inflammatory sites requires intercellular adhesion.
- Leukocyte adherence involves rolling, stopping, and transmigration through endothelial cells.
- Adhesion molecules for rolling may differ from those for stopping and transmigration.
Purpose of the Study:
- To investigate the role of E-selectin (ELAM-1) in neutrophil adhesion and rolling.
- To assess the efficacy of anti-E-selectin monoclonal antibody in blocking these processes.
Main Methods:
- In vitro adhesion assay using isolated human neutrophils and murine L-cell monolayers transfected with human E-selectin cDNA.
- Application of physiological wall shear stress (1.85 dynes/cm2).
- Use of anti-E-selectin, anti-L-selectin, and anti-CD18 monoclonal antibodies.
Main Results:
- Human neutrophils adhered to and rolled on E-selectin expressing L-cells, an interaction blocked by anti-E-selectin antibody.
- Anti-L-selectin antibody reduced adhesion to E-selectin expressing cells by 70%.
- Chemotactic stimulation affected rolling cell numbers and velocity but not the percentage of rolling cells.
Conclusions:
- E-selectin (ELAM-1) is a key mediator of neutrophil rolling adhesion under specific in vitro conditions.
- Anti-E-selectin antibodies effectively inhibit this initial phase of neutrophil adhesion.
- The role of E-selectin in neutrophil adhesion to human endothelial cells under flow is less pronounced.