Quantification of chicken alpha-fetoprotein: a useful tool in studies of embryo development and pathology

Chicken alpha-fetoprotein (AFP) from the plasma of 12-day-old chick embryos was purified by electroelution from SDS/PAGE gels, and used to produce polyclonal and monoclonal antibodies. Both reagents were then used to design a sandwich-type enzyme-immunoassay for the quantification of AFP in biological fluids. The assay was used to quantify AFP in the serum and amniotic fluid of chick embryos with abnormalities of the neural tube. Serum AFP was significantly greater in these embryos than in normal ones of similar age. Moreover, substantial amounts of AFP were demonstrated in the amniotic fluid, whereas this protein was undetectable in the amniotic fluid of normal embryos. This method of assay may provide a reliable tool for studies of chick embryogenesis and abnormalities of embryonal differentiation.