Characterization and expression of the Xenopus c-Myb homolog

The c-Myb protein is a sequence specific DNA-binding transcriptional regulator that is critically involved in the regulation of hematopoietic differentiation. Its role in these processes suggests that the function of c-Myb may be important early in the establishment of the hematopoietic lineage. We have isolated cDNA and partial genomic clones representing the Xenopus c-Myb homolog (Xc-Myb) in order to examine the role this gene plays in early mesodermal patterning in the frog embryo. The establishment of these clones as c-Myb homologs, as opposed to Myb-related sequences, is based upon both predicted amino acid sequences and the location of the exon-intron boundaries within the Xc-Myb gene. Maternally derived Xc-Myb RNA is degraded following fertilization then, beginning at midblastula, re-accumulates throughout early development. Xc-Myb RNA is localized to the animal cap region of the early blastula. Following the onset of gastrulation expression predominates in the ventral half of the embryo. During neurulation expression of Xc-Myb is observed in both the anterior dorsal and ventral vegetal regions of the embryo. Expression of Xc-Myb occurs in several adult tissues, the highest levels of which are in the intestine, heart, liver, lung and ovary. Xc-Myb encodes a protein of 624 amino acids and exhibits a mobility in SDS-PAGE of approximately 75 kDa, identical with that of the murine c-Myb protein. Xc-Myb protein exhibits 70% identity with avian and 67% identity with mammalian c-Myb proteins.