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Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an
1Institute of Microbiology, Czech Academy of Sciences, Prague.
Yeast (Chichester, England)
|November 1, 1993
Abstract:
A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5.2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.