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[Distribution and function of gp90MEL-14 positive and negative T lymphocytes]
1Section of Pathology, Hokkaido University, Sapporo, Japan.
[Hokkaido Igaku Zasshi] the Hokkaido Journal of Medical Science
|November 1, 1993
Summary
The addition of NaN3 stabilizes gp90MEL-14 molecule expression on lymphocytes, enabling accurate measurement. This revealed that gp90MEL-14 negative T cells are functionally active and exhibit enhanced immune responses compared to gp90MEL-14 positive T cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- Lymphocyte surface molecule expression, such as gp90MEL-14, is crucial for immune cell function but can be unstable during in vitro processing.
- Understanding lymphocyte subset function requires reliable methods to quantify cell surface markers and assess their associated immune activity.
Purpose of the Study:
- To develop a method for stabilizing gp90MEL-14 molecule expression on lymphocytes for accurate quantification.
- To investigate the in vivo distribution and functional significance of gp90MEL-14 expressing T cells in peripheral lymphoid tissues.
Main Methods:
- Addition of sodium azide (NaN3) to cell suspension media to stabilize gp90MEL-14 expression.
- Flow cytometry to quantify gp90MEL-14 and Pgp-1 expression on lymphoid cells.
- In vitro functional assays including mixed lymphocyte reaction, concanavalin A stimulation, and cytokine production (IL-2, IFN-gamma) to compare gp90MEL-14 positive and negative T cell subsets.
Main Results:
- Sodium azide (NaN3) addition stabilized gp90MEL-14 expression, allowing precise quantification and reflecting in vivo patterns.
- gp90MEL-14 expression correlated with anatomical and functional distance from high endothelial venules and inversely with Pgp-1 expression.
- gp90MEL-14 negative CD8+ T cells exhibited significantly higher proliferative responses and produced more IL-2 and IFN-gamma than gp90MEL-14 positive CD8+ T cells.
- gp90MEL-14 negative CD4+ T cells showed slightly higher IL-2 secretion but similar proliferation compared to gp90MEL-14 positive CD4+ T cells.
Conclusions:
- A simple method using NaN3 stabilizes lymphocyte gp90MEL-14 expression for accurate in vivo assessment.
- gp90MEL-14 negative T cells, particularly CD8+ T cells, represent a functionally active subpopulation in peripheral lymphoid tissues.
- These findings suggest that gp90MEL-14 negative T cells may play distinct roles in immune responses compared to the major gp90MEL-14 positive T cell population.