Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A bioassay based on recombinant DNA technology for determining selenium concentration

M Reches1, C Zhao, H Engelberg-Kulka

  • 1Department of Molecular Biology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.

Applied and Environmental Microbiology
|January 1, 1994
PubMed
Summary

Researchers developed a novel bioassay using recombinant DNA technology to detect trace selenium. This method leverages the unique incorporation of selenocysteine, the 21st amino acid, to quantify selenium derivatives.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Tensor, Vector, and Scalar Polarizations in the Stochastic Gravitational-Wave Background.

Physical review letters·2018
Same author

Prospects for observing and localizing gravitational-wave transients with Advanced LIGO, Advanced Virgo and KAGRA.

Living reviews in relativity·2018
Same author

Effects of early comprehensive interventions on child neurodevelopment in poor rural areas of China: a moderated mediation analysis.

Public health·2018
Same author

D-Meson Azimuthal Anisotropy in Midcentral Pb-Pb Collisions at sqrt[s]_{NN}=5.02  TeV.

Physical review letters·2018
Same author

GW170817: Implications for the Stochastic Gravitational-Wave Background from Compact Binary Coalescences.

Physical review letters·2018
Same author

Increased circulating microparticles in women with preeclampsia.

International journal of laboratory hematology·2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Selenium is a trace element crucial for human health due to its role in enzyme active sites.
  • Selenocysteine, the 21st amino acid, is incorporated into proteins via UGA codons, expanding the genetic code.
  • Existing methods for trace selenium detection can be limited.

Purpose of the Study:

  • To develop a sensitive bioassay for detecting simple selenium derivatives.
  • To utilize recombinant DNA technology for trace element detection.
  • To establish a reporter system based on UGA-directed selenocysteine incorporation.

Main Methods:

  • Construction of recombinant DNA plasmids in Escherichia coli.
  • Expression of the lac'Z gene driven by UGA-directed selenocysteine incorporation.

Related Experiment Videos

  • Measurement of beta-galactosidase activity as an indicator of selenium presence.

    • Main Results:

    • Beta-galactosidase activity was proportionally and specifically related to selenium derivative concentrations.
    • The developed system demonstrated sensitivity in detecting trace selenium.
    • Recombinant DNA technology was successfully employed for chemical trace element detection.

    Conclusions:

    • A novel and sensitive bioassay for simple selenium derivatives has been established.
    • This bioassay utilizes the expanded genetic code and UGA-directed selenocysteine incorporation.
    • The system offers a new approach for trace element determination using recombinant DNA technology.