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Related Experiment Videos

CD40L: a multi-functional ligand

R J Armitage1, C R Maliszewski, M R Alderson

  • 1Department of Cellular Immunology, Immunex Research and Development Corporation, Seattle, WA 98101.

Seminars in Immunology
|December 1, 1993
PubMed
Summary
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Activated T cells signal B cells to grow and change using CD40 ligand. This interaction is crucial for immunoglobulin heavy chain switching and producing various antibody types, impacting multiple immune cell types.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • B cell activation requires cell-contact dependent signals from T cells.
  • Antibodies targeting CD40 mimic T cell signals for B cell activation in humans.
  • CD40 ligand was identified as a key mediator of T cell-dependent B cell stimulation.

Purpose of the Study:

  • To elucidate the role of CD40 ligand in B cell activation and differentiation.
  • To investigate the necessity of CD40 signaling for immunoglobulin class switching and production.
  • To explore the broader functions of CD40 ligand beyond B cell interactions.

Main Methods:

  • Cloning of the CD40 ligand gene.
  • Development of blocking reagents to inhibit CD40-CD40 ligand interaction.

Related Experiment Videos

  • Studies on individuals with non-functional CD40 ligand.

    • Main Results:

    • CD40 ligand is the primary contact-dependent signal for B cell activation.
    • CD40 signaling is essential for immunoglobulin heavy chain switching and production of all isotypes except IgM.
    • CD40 ligand also stimulates cells of monocyte and T cell lineages.

    Conclusions:

    • CD40 ligand plays a critical role in adaptive immunity, particularly in B cell antibody production.
    • CD40 signaling is indispensable for immunoglobulin class switching beyond IgM.
    • CD40 ligand exhibits pleiotropic effects, influencing multiple immune cell types in vivo.