Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rous sarcoma virus-transformed cells develop peculiar adhesive structures along the cell periphery

N Nakamura1, J Tanaka, K Sobue

  • 1Department of Neurochemistry and Neuropharmacology, Osaka University Medical School, Japan.

Journal of Cell Science
|December 1, 1993
PubMed
Summary

Researchers discovered a new cell adhesion structure, peripheral adhesions, in Rous sarcoma virus-transformed fibroblasts. These structures, distinct from focal adhesions and podosomes, involve integrins and may play a role in cell adhesion for cells with poor stress fiber organization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Specificity of androgenic regulation of input to sexually dimorphic motoneurons in male rat lumbar spinal cord.

Experimental neurology·1995
Same author

Serum levels of six pancreatic enzymes as related to the degree of renal dysfunction.

The American journal of gastroenterology·1995
Same author

Coronary artery reoperation through the left thoracotomy with hypothermic circulatory arrest.

The Annals of thoracic surgery·1995
Same author

Possible role of T-cell surface molecule gene expression in the mixed lymphocyte culture for predicting transplantation-related complications after allogeneic bone marrow transplantation.

European journal of haematology·1995
Same author

Monoethylglycinexylidide formation as an independent measure of warm hepatic ischemia and reperfusion injury.

The Journal of surgical research·1995
Same author

Laparoscopic retrieval of disconnected ventriculoperitoneal shunt catheters: report of two cases.

Surgical laparoscopy & endoscopy·1995

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cellular transformation by Rous sarcoma virus (RSV) alters cell/substratum adhesive structures.
  • Fibroblasts exhibit various adhesive structures, including focal adhesions and podosomes.

Purpose of the Study:

  • To investigate the alterations in cell adhesive structures of rat fibroblasts (3Y1 cells) after transformation by Rous sarcoma virus (RSV).
  • To characterize a novel adhesive structure, termed peripheral adhesions, and compare it with known structures like focal adhesions and podosomes.

Main Methods:

  • Immunofluorescence microscopy was employed to visualize cell adhesive structures.
  • Interference reflection microscopy was used to observe peripheral adhesions and podosomes.
  • Treatment with RGD-containing peptides assessed the role of integrins in adhesion.

Related Experiment Videos

Main Results:

  • RSV-transformed fibroblasts (BY1 cells) exhibited peripheral adhesions, characterized by integrin localization and distinct from focal adhesions and podosomes.
  • Peripheral adhesions lacked actin bundles but accumulated pp60v-src and tyrosine-phosphorylated proteins.
  • These adhesions were sensitive to RGD-containing peptides and were observed in various cell types with compromised stress fiber organization.

Conclusions:

  • Peripheral adhesions represent a novel class of stable adhesive structures, distinct from focal adhesions and podosomes.
  • These structures are mediated by integrins and are prevalent in cells with poorly developed focal adhesions and stress fibers.
  • Peripheral adhesions may play a significant role in the adhesion of various cell types, particularly under conditions of altered cytoskeletal organization.