DNA strandness changes during in situ hybridization conditions
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1Istituto di Biologia, Facoltà di Medicina, Università di Cagliari, Italy.
European Journal of Histochemistry : EJH
|January 1, 1993
Abstract:
DNA strandness changes occurring during in situ hybridization were monitored using monoclonal antibodies. Our results show that: 1) RNase induces formation of single stranded DNA, 2) after denaturation-renaturation, single stranded DNA is found principally in centromeric areas, and 3) double stranded DNA is still observed after each step.