Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for isolating alpha 2 macroglobulin-cytokine complexes

A J Cunningham1, S F Elliott, J R Black

  • 1Department of Surgery (WGH), University of Edinburgh Medical School, UK.

Journal of Immunological Methods
|March 10, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observations on Curative Evidence.

Atlanta medical and surgical journal·2022
Same author

Chronic Pharyngitis.

Atlanta medical and surgical journal·2022
Same author

Two Cases of Sprue (Psilosis, Dr. Thin).

Glasgow medical journal·2018
Same author

Radical Chain Reduction of CCl<sub>4</sub> Initiated by Illumination of SPEEK Solutions.

The journal of physical chemistry. A·2017
Same author

Low birth weight and intelligence in adolescence and early adulthood: a meta-analysis.

Journal of public health (Oxford, England)·2013
Same author

Preoperative tests: an Irish perspective.

Irish journal of medical science·2011
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

Researchers developed a new zinc-affinity chromatography method to isolate alpha 2 macroglobulin-cytokine complexes. This technique helps investigate the immunological role of cytokine binding by alpha 2 macroglobulin (alpha 2-M).

Area of Science:

  • Biochemistry
  • Immunology
  • Proteomics

Background:

  • Cytokine-binding by alpha 2 macroglobulin (alpha 2-M) is crucial for immune regulation.
  • Investigating these complexes requires methods to separate them from free cytokines.

Purpose of the Study:

  • To develop a simple and effective method for separating alpha 2 macroglobulin-cytokine complexes from free cytokines.
  • To facilitate further research into the nature and immunological significance of cytokine binding by alpha 2-M.

Main Methods:

  • Utilized zinc-binding sites present on alpha 2-M.
  • Employed zinc-affinity chromatography for protein isolation.
  • The method can be applied in both column and batch formats.

Main Results:

Related Experiment Videos

  • Successfully isolated alpha 2 macroglobulin-cytokine complexes.
  • Demonstrated the effectiveness of zinc-affinity chromatography for this separation.
  • Validated a versatile method applicable in different formats.

Conclusions:

  • A novel and efficient method for isolating alpha 2 macroglobulin-cytokine complexes has been established.
  • This technique provides a valuable tool for studying cytokine-alpha 2-M interactions.
  • Further investigation into the immunological significance of these complexes is now feasible.