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Related Experiment Videos

Expression of the prostate-specific membrane antigen

R S Israeli1, C T Powell, J G Corr

  • 1Urologic Oncology Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

Cancer Research
|April 1, 1994
PubMed
Summary
This summary is machine-generated.

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Researchers cloned the prostate-specific membrane antigen (PSM) gene. PSM is highly expressed in prostate cancer and normal tissues, with expression modulated by hormones.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • The prostate-specific membrane antigen (PSM) is a target recognized by the 7E11-C5.3 anti-prostate monoclonal antibody.
  • Understanding PSM expression is crucial for prostate cancer research and diagnostics.

Purpose of the Study:

  • To clone and characterize the complementary DNA (cDNA) encoding PSM.
  • To investigate PSM expression in various prostate cancer cell lines and human tissues.
  • To explore the hormonal regulation of PSM expression.

Main Methods:

  • Complementary DNA (cDNA) cloning and in vitro transcription/translation.
  • Immunohistochemical analysis and Western blotting for PSM detection.
  • Ribonuclease protection assays for PSM mRNA expression.

Related Experiment Videos

  • Hormonal modulation studies using steroids in cell lines and xenograft models.

    • Main Results:

    • The full-length PSM cDNA was cloned, encoding an M(r) 84,000 precursor protein that is post-translationally modified to an M(r) 100,000 glycoprotein.
    • PSM was intensely expressed in LNCaP cells but not in DU-145 or PC-3 cells; expression was confirmed in transfected PC-3 cells.
    • PSM mRNA expression is prostate-specific, with highest levels in hormone-deprived states.
    • Steroids, including 5-alpha-dihydrotestosterone and testosterone, significantly down-regulated PSM expression in LNCaP cells.
    • Normal and malignant prostatic tissues showed high PSM expression, while benign prostatic hyperplasia exhibited heterogeneous or absent expression.
    • LNCaP tumors in nude mice models demonstrated abundant PSM expression.

    Conclusions:

    • The cloned PSM cDNA provides a tool to study PSM expression and regulation.
    • PSM is a prostate-specific antigen with expression modulated by hormones, making it a potential therapeutic and diagnostic target.
    • The characterized PSM expression patterns in cell lines and tissues, along with the in vivo model, offer valuable insights for future research.