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Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Published on: July 10, 2013
Development of surface antigen during maturation of bone marrow neutrophil granulocytes
Abstract:
The presence of neutrophil surface antigens on maturing bone marrow granulocytes was examined by indirect immunofluorescence and cellular immunoadsorption using heterologous antibody to mature neutrophil granulocytes. The results show that bone marrow neutrophils possess surface antigens that appear during cell maturation from myeloblasts. Fluorescent antibody capping and patching, indicators of antigen mobility, were more pronounced in mature than in immature cells. Neutrophil surface antigen development could also be demonstrated during granulocyte maturation in vitro.
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