Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

60-kDa Ro protein autoepitopes identified using recombinant polypeptides

M R Saitta1, F C Arnett, J D Keene

  • 1Department of Microbiology, Duke University Medical Center, Durham, NC 27710.

Journal of Immunology (Baltimore, Md. : 1950)
|April 15, 1994
PubMed
Summary

Autoantibodies targeting the 60-kDa Ro protein can recognize discontinuous epitopes, especially when anti-52-kDa Ro antibodies are present. This finding clarifies the heterogeneity of Ro autoantibody responses in autoimmune diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

One-hundred-fifteen consecutive bladder exstrophies successfully closed in a single nationally commissioned centre.

Journal of pediatric urology·2025
Same author

Attenuation of expression of extracellular matrix genes with siRNAs to Sparc and Ctgf in skin fibroblasts of CTGF transgenic mice.

International journal of immunopathology and pharmacology·2011
Same author

Clinical presentations and molecular basis of complement C1r deficiency in a male African-American patient with systemic lupus erythematosus.

Lupus·2011
Same author

Analysis of the influence of PTPN22 gene polymorphisms in systemic sclerosis.

Annals of the rheumatic diseases·2010
Same author

The FAS -670A>G polymorphism influences susceptibility to systemic sclerosis phenotypes.

Arthritis and rheumatism·2009
Same author

BANK1 functional variants are associated with susceptibility to diffuse systemic sclerosis in Caucasians.

Annals of the rheumatic diseases·2009

Area of Science:

  • Immunology
  • Autoimmunity
  • Molecular Biology

Background:

  • The human Ro ribonucleoprotein is a key autoantigen in autoimmune diseases, but its role in pathogenesis and the triggers for immune response are not fully understood.
  • Ro autoantibodies exhibit heterogeneity, targeting specific proteins (60-kDa and 52-kDa) and various epitopes, complicating their diagnostic and prognostic value.

Purpose of the Study:

  • To investigate the heterogeneity of autoantibody recognition of the 60-kDa Ro protein, focusing on discontinuous epitopes.
  • To correlate autoantibody reactivity patterns with clinical diagnosis, autoantibody titers, and the presence of antibodies against the 52-kDa Ro protein.

Main Methods:

  • Generation of 10 overlapping recombinant polypeptides of the human 60-kDa Ro protein.
  • Comparison of autoantibody reactivities using a soluble immunoprecipitation assay with sera from 12 patients.

Related Experiment Videos

  • Analysis of antibody recognition patterns, including continuous and discontinuous epitopes.

    • Main Results:

    • Seven distinct epitopes (continuous and discontinuous) and seven reactivity patterns were identified among patient sera.
    • Reactivity patterns did not correlate with clinical diagnosis but were associated with anti-60 kDa Ro antibody titers and the presence of anti-52 kDa Ro antibodies.
    • Sera recognizing only full-length 60-kDa protein, particularly discontinuous epitopes, showed low anti-60 kDa titers by ELISA and immunoblot.

    Conclusions:

    • Autoantibody responses to the 60-kDa Ro antigen can preferentially target discontinuous epitopes.
    • Recognition of continuous epitopes on 60-kDa Ro is associated with the presence of autoantibodies against the 52-kDa Ro protein.