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Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe
1Department of Pediatrics and Child Health, Manitoba Institute of Cell Biology, Winnipeg, Canada.
Connective Tissue Research
|January 1, 1993
Abstract:
A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.