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Updated: May 15, 2026

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
Published on: October 21, 2017
Interferon-gamma and interleukin-1 beta induce nitric oxide formation from primary mouse astrocytes
S J Hewett1, J A Corbett, M L McDaniel
1Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
Abstract:
An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1 beta (IL1 beta) plus interferon-gamma (INF gamma) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor NG-nitro-L-arginine and the protein synthesis inhibitor cycloheximide.

