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Extraction of Tissue Antigens for Functional Assays
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In vitro primary responses of human T cells to soluble protein antigens

M Plebanski1, S S Burtles

  • 1Department of Pathology and Microbiology, Medical School, University of Bristol, UK.

Journal of Immunological Methods
|March 29, 1994
PubMed

The optimum culture conditions to support in vitro proliferative responses to conventional soluble protein antigens by human CD4+ T cells from healthy non-immunised donors have been determined. These responses were blocked by anti-HLA-DR monoclonal antibodies (mAbs) and were strictly dependent on the presence of antigen-presenting cells (APCs). Primary responses showed a reproducible pattern of proliferation kinetics which distinguished them from secondary in vitro T cells reactions. T cells specific for the sensitising antigen were recovered from primary cultures.

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