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Related Experiment Videos

Canine galanin: sequence, expression and pancreatic effects

M R Boyle1, C B Verchere, G McKnight

  • 1Department of Medicine, University of Washington, Seattle 98108.

Regulatory Peptides
|February 3, 1994
PubMed
Summary

Dog galanin, a newly identified peptide, potently inhibits insulin secretion and is expressed in nerves innervating the pancreas, suggesting a role in sympathetic neurotransmission.

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Area of Science:

  • Endocrinology
  • Neuroscience
  • Molecular Biology

Background:

  • Galanin is a neuropeptide found in various tissues, including the pancreas.
  • Its role in regulating insulin secretion and its specific form in dogs were not well understood.

Purpose of the Study:

  • To elucidate the primary structure and pancreatic expression of dog galanin.
  • To investigate the effects of dog galanin on pancreatic hormone secretion.

Main Methods:

  • Determined the primary structure of dog galanin from cloned cDNA.
  • Evaluated dog progalanin mRNA expression in celiac ganglia using in situ hybridization.
  • Synthesized dog galanin peptide and administered it intravenously to dogs.
  • Measured insulin, somatostatin, and glucagon secretion in response to galanin infusion.

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Main Results:

  • The deduced amino acid sequence of dog galanin differs from other species.
  • Dog progalanin mRNA was detected in all neuronal cell bodies within the celiac ganglion.
  • Intravenous infusion of dog galanin potently inhibited insulin secretion.
  • Galanin also inhibited somatostatin release and stimulated glucagon secretion.
  • The C-terminal portion of dog galanin retains potent insulin inhibitory activity.

Conclusions:

  • Dog galanin is expressed in neurons innervating the canine pancreas.
  • The structure of dog galanin supports its role in regulating insulin secretion.
  • These findings support the hypothesis that galanin acts as a sympathetic neurotransmitter in the dog pancreas.