Related Experiment Videos
Preservation of rat bone marrow with dimethylsulphoxide at -150 degrees C
Abstract:
The authors tested preserving properties of three concentrations of dimethylsulphoxide (15%, 10% and 7.5%) in preservation of rat bone marrow cells at -150 degrees C. Cells of rat bone marrow were frozen at 1 degree C/min to -20 degrees C, 5 degrees C/min to -80 degrees C and then placed directly at -150 degrees C and held at such temperature for 6 months. Vitality of cells was checked monthly for a period of 6 months by means of several vitality tests with dyes (eosin and trypane blue), autoradiography and erythrophagocytosis. It was found that cells capable of cleavage could be equally preserved at such low temperature with all the three DMSO concentrations while mature cells (granulocytes, reticular cells) revealed considerably higher erythrophagocytic activity when preserved at 15% DMSO and lower activity at 10% and 7.5% DMSO.
Insights
Dimethyl sulfoxide (DMSO) effectively preserves rat bone marrow cells at -150°C for six months. Higher DMSO concentrations (15%) enhanced erythrophagocytic activity in mature cells.
Area of Science:
- Cryobiology
- Cell Biology
- Hematology
Background:
- Cryopreservation of biological samples is crucial for long-term storage.
- Dimethyl sulfoxide (DMSO) is a widely used cryoprotective agent.
- Optimizing DMSO concentrations is essential for maintaining cell viability and function.
Purpose of the Study:
- To evaluate the efficacy of three different concentrations of dimethyl sulfoxide (DMSO) for cryopreserving rat bone marrow cells.
- To assess the impact of DMSO concentration on cell viability and functional activity after prolonged storage at -150°C.
Main Methods:
- Rat bone marrow cells were cryopreserved using 15%, 10%, and 7.5% DMSO.
- Controlled cooling rates were applied: 1°C/min to -20°C, then 5°C/min to -80°C, followed by direct transfer to -150°C.
- Cell vitality was assessed monthly for six months using eosin and trypan blue staining, autoradiography, and erythrophagocytosis assays.
Main Results:
- All tested DMSO concentrations (15%, 10%, 7.5%) supported the preservation of cells capable of cleavage.
- Mature cells, including granulocytes and reticular cells, exhibited significantly higher erythrophagocytic activity when preserved with 15% DMSO.
- Lower DMSO concentrations (10% and 7.5%) resulted in reduced erythrophagocytic activity in mature cells.
Conclusions:
- Dimethyl sulfoxide (DMSO) at concentrations of 7.5% to 15% is effective for cryopreserving rat bone marrow cells at -150°C.
- A 15% DMSO concentration appears optimal for preserving the erythrophagocytic function of mature bone marrow cells.
- Further research may explore the long-term functional implications of different DMSO concentrations on various cell types.