[Affinity chromatography of proteinases]

Studies on the affinity chromatography of proteinases as the most efficient approach to their separation are reviewed. The paper contains discussion of the methods used to prepare affinity sorbents by attaching gramicidin and bacitracin to various supports. The two cyclopeptides contain amino acid residues, meeting specificity requirements of proteinases of various classes, these materials thus being affinity sorbents of general type. Rules governing the interaction of proteinases with these sorbents are discussed, along with numerous examples of their chromatography on sorbents of general type as well as on more specific sorbents adapted to the separation of particular types of proteinases. Sorbents containing benzylsuccinic, benzylmalonic and phenylboronic acid residues are considered in details.