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Related Experiment Videos

Developmental expression of human microsomal epoxide hydrolase

C J Omiecinski1, L Aicher, L Swenson

  • 1Department of Environmental Health, University of Washington, Seattle.

The Journal of Pharmacology and Experimental Therapeutics
|April 1, 1994
PubMed
Summary

Microsomal epoxide hydrolase (mEH) levels increase with gestational age in fetal liver, suggesting early fetuses are more vulnerable to epoxide toxins. Post-transcriptional regulation impacts enzyme activity.

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Area of Science:

  • Biochemistry
  • Toxicology
  • Developmental Biology

Background:

  • Microsomal epoxide hydrolase (mEH) is crucial for detoxifying epoxides from drugs and environmental agents.
  • mEH plays a role in chemically induced cancers and developmental toxicities.

Purpose of the Study:

  • To characterize mEH expression in human tissues across gestational ages.
  • To assess fetal risk from developmental toxins by evaluating mEH levels.

Main Methods:

  • Enzymatic activity assays.
  • Immunochemical protein quantitation.
  • RNA hybridization assays.

Main Results:

  • Hepatic mEH activity strongly correlated with gestational age (r = .82) and protein levels (r = .93).

Related Experiment Videos

  • Fetal lung mEH activity and RNA levels did not correlate with gestational age or activity.
  • Liver and adrenal glands showed highest mEH RNA; lung and kidney showed lower levels.

    • Conclusions:

    • Post-transcriptional regulation likely influences mEH functional activity.
    • Fetuses in early gestation may be more sensitive to epoxide xenobiotics than those at later stages.