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Methods to study peptides associated with MHC class I molecules

S Joyce1, S G Nathenson

  • 1Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Milton S Hershey Medical Center, Hershey.

Current Opinion in Immunology
|February 1, 1994
PubMed

It is now an accepted fact that peptides of self or non-self origin form an essential component of the MHC class I structure. The peptide component of the heterotrimer contains the essential determinants recognized by the T-cell receptors of cytotoxic T lymphocytes, be it an antigen-specific, alloimmune or autoimmune response. Because of the importance of the recognition process, several methods have been developed to characterize naturally processed peptides presented by the class I molecules.

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...