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Updated: Sep 25, 2026
Preparing T Cell Growth Factor from Rat Splenocytes
Published on: November 1, 2007
Reduction of tumor necrosis factor production by splenocytes from v-Ha-ras oncogene-bearing mice
G J Chen1, L L Colombo, M C Lopez
1Department of Family and Community Medicine, University of Arizona, Tucson 85624.
Abstract:
Mice containing the activated v-Ha-ras oncogene driven by the mouse mammary tumor virus promoter/enhancer produced less tumor necrosis factor (TNF) than genetically identical animals without it. Inbred Oncomice containing the v-Ha-ras oncogene and inbred FVB mice without it were grown for 6 months. Splenocytes were isolated and stimulated in vitro to produce tumor necrosis factor (TNF) and gamma-interferon (IFN). TNF production by cells from Oncomice was significantly decreased compared to cells from FBV mice. There was a tendency for decrease, but no significant difference was seen on IFN release. These observations suggest that the oncogene may play a role in the immune system.
Insights
Mice with the v-Ha-ras oncogene showed reduced tumor necrosis factor (TNF) production. This suggests oncogenes may impact the immune system, affecting cytokine release like TNF.
Area of Science:
- Immunology
- Oncology
- Molecular Biology
Background:
- The v-Ha-ras oncogene is implicated in various cancers.
- Cytokine production, such as tumor necrosis factor (TNF), is crucial for immune responses.
- The interaction between oncogenes and immune function requires further investigation.
Purpose of the Study:
- To investigate the effect of the activated v-Ha-ras oncogene on cytokine production in mice.
- To compare TNF and gamma-interferon (IFN) levels in Oncomice versus control mice.
Main Methods:
- Inbred Oncomice (carrying v-Ha-ras) and FVB mice (control) were studied over 6 months.
- Splenocytes were isolated and stimulated in vitro.
- Quantification of TNF and IFN release was performed.
Main Results:
- Splenocytes from Oncomice produced significantly less TNF compared to control FVB mice.
- A non-significant trend towards decreased IFN release was observed in Oncomice.
- These findings indicate a specific impact of the v-Ha-ras oncogene on TNF production.
Conclusions:
- The activated v-Ha-ras oncogene is associated with decreased TNF production in mice.
- Oncogenes may play a regulatory role in the immune system by modulating cytokine release.
- Further research is warranted to elucidate the mechanisms underlying oncogene-mediated immune modulation.
