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PMN adherence to cat ischemic-reperfused mesenteric vascular endothelium under flow: role of P-selectin
1Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-6799.
Abstract:
We studied polymorphonuclear leukocyte (PMN) adherence to cat ischemic-reperfused mesenteric artery and vein endothelia under conditions of flow in vitro. Under physiological shear rates, only a few PMNs adhered to non-ischemic-reperfused arterial and venous endothelia (11 +/- 2 and 20 +/- 3 PMN/mm2, respectively). However, after 60 min of ischemia and 20 or 120 min of reperfusion, a significant increase in PMN adherence to arterial endothelium was observed. At 20 min of reperfusion, 44 +/- 4 PMN/mm2 adhered, and at 120 min postreperfusion, 63 +/- 12 PMN/mm2 adhered (P < 0.01 from control). Moreover, a greater degree of PMN adherence occurred on the venous than on the arterial endothelium. Thus, 159 +/- 10 and 198 +/- 12 PMN/mm2 adhered to mesenteric venous endothelium isolated after 20 and 120 min reperfusion, respectively (P < 0.01 vs. arteries). Furthermore, addition of PB 1.3 (20 micrograms/ml), a monoclonal antibody against P-selectin, 5 min before perfusion with PMNs significantly attenuated the increase in PMN adherence to both arterial and venous endothelia (P < 0.01). These results indicate that PMN-endothelial interaction also occurs in conduit vessels after ischemia and reperfusion, although a more profound PMN adherence occurs in veins.
Insights
Polymorphonuclear leukocyte (PMN) adherence increases significantly in cat mesenteric arteries and veins after ischemia and reperfusion. This PMN-endothelial interaction is more pronounced in veins and can be reduced by blocking P-selectin.
Area of Science:
- Vascular Biology
- Immunology
- Surgical Research
Background:
- Ischemia and reperfusion injury can lead to significant vascular damage.
- Polymorphonuclear leukocytes (PMNs) play a critical role in the inflammatory response following such events.
Purpose of the Study:
- To investigate PMN adherence to cat mesenteric artery and vein endothelium after ischemia and reperfusion.
- To determine the role of P-selectin in PMN-endothelial interactions in conduit vessels.
Main Methods:
- In vitro study using cat mesenteric arteries and veins subjected to ischemia and reperfusion.
- Measurement of PMN adherence under physiological flow conditions.
- Use of a monoclonal antibody (PB 1.3) against P-selectin to assess its role.
Main Results:
- PMN adherence significantly increased on both arterial and venous endothelium after ischemia and reperfusion.
- Venous endothelium exhibited a greater degree of PMN adherence compared to arterial endothelium.
- Blocking P-selectin with PB 1.3 antibody significantly reduced PMN adherence to both vessel types.
Conclusions:
- PMN-endothelial interactions occur in conduit vessels following ischemia and reperfusion.
- Venous endothelium is more susceptible to PMN adherence than arterial endothelium post-insult.
- P-selectin is a key mediator in PMN recruitment to the endothelium in this model.