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Related Experiment Videos

Characterization of regulatory volume decrease in the THP-1 and HL-60 human myelocytic cell lines

E K Gallin1, T M Mason, A Moran

  • 1Department of Physiology, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20889-5603.

Journal of Cellular Physiology
|June 1, 1994
PubMed
Summary
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Regulatory volume decrease (RVD) in THP-1 and HL-60 cells involves separate anion and cation transport pathways. These pathways are activated by hypotonic stress and are quinine-inhibitable and DISA-sensitive, respectively.

Area of Science:

  • Cellular Physiology
  • Membrane Transport
  • Volume Regulation

Background:

  • Hypotonic stress triggers cell volume changes, necessitating regulatory volume decrease (RVD) for cell survival.
  • THP-1 and HL-60 cells exhibit RVD, but the underlying ion transport mechanisms require detailed investigation.

Purpose of the Study:

  • To elucidate the specific ion transport mechanisms involved in regulatory volume decrease (RVD) in THP-1 and HL-60 cells under hypotonic stress.
  • To determine the role of cation and anion channels in RVD and their sensitivity to specific blockers.

Main Methods:

  • Exposure of THP-1 and HL-60 cells to hypotonic stress in varying ionic solutions.
  • Assessment of cell volume changes and application of ion channel blockers like quinine, barium, and 3,5-diiodosalicylic acid (DISA).

Related Experiment Videos

  • Investigation of calcium-independent pathways and anion permeability using different anions.

    • Main Results:

    • RVD requires an outward potassium gradient; failure to regulate leads to secondary swelling.
    • Quinine and 3,5-diiodosalicylic acid (DISA) inhibit RVD by blocking cation and anion pathways, respectively.
    • RVD is independent of calcium-activated conductances and involves a swelling-induced anion efflux pathway permeable to chloride and bromide.

    Conclusions:

    • Regulatory volume decrease in THP-1 and HL-60 cells is mediated by distinct anion and cation transport mechanisms.
    • The identified pathways include a DISA-sensitive anion pathway and a quinine-inhibitable potassium efflux pathway.
    • Neither pathway requires an increase in intracellular calcium for activation.