Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Virus-specific CD8+ T-cell memory determined by clonal burst size

S Hou1, L Hyland, K W Ryan

  • 1Department of Immunology, St Jude Children's Research Hospital, Memphis, Tennessee 38105.

Nature
|June 23, 1994
PubMed
Summary

Virus-specific CD8+ T-cell memory persists long-term without continuous viral antigen exposure. This study shows memory T-cells are maintained independently of persistent viral peptide sources.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunity to avian influenza A viruses.

Revue scientifique et technique (International Office of Epizootics)·2009
Same author

Thinking about broadly cross-reactive vaccines.

Clinical pharmacology and therapeutics·2009
Same author

Visualizing CTL activity for different CD8+ effector T cells supports the idea that lower TCR/epitope avidity may be advantageous for target cell killing.

Cell death and differentiation·2009
Same author

Crystal-ball gazing--the future of immunological research viewed from the cutting edge.

Clinical and experimental immunology·2006
Same author

Endings and beginnings.

Cellular and molecular life sciences : CMLS·2006
Same author

Neuraminidase inhibitors as antiviral agents.

Current drug targets. Infectious disorders·2006

Area of Science:

  • Immunology
  • Virology
  • T-cell memory research

Background:

  • Some viruses establish lifelong infections, while others like influenza A, require periodic boosting of CD8+ T-cell immunity.
  • Prolonged CD8+ T-cell memory is observed for viruses not typically re-encountered or persisting in the host genome.
  • This suggests CD8+ T-cell memory may not depend on continuous or intermittent antigen exposure.

Purpose of the Study:

  • To investigate the mechanisms maintaining long-term CD8+ T-cell memory.
  • To determine if persistent viral antigen is essential for sustaining virus-specific CD8+ T-cell memory.
  • To assess the longevity of CD8+ T-cell memory in the absence of detectable viral peptide sources.

Main Methods:

  • Adoptive transfer of virus-specific CD8+ memory T cells into naive and previously infected recipient mice.

Related Experiment Videos

  • Restimulation assays with viral antigens to assess clonal expansion and memory cell detection.
  • Analysis of T-cell maintenance in irradiated and beta 2-microglobulin-deficient mice.
  • Detection of viral antibodies and peptides in recipient mice to assess antigen persistence.

    • Main Results:

    • Restimulation with Sendai virus antigen significantly increased memory cytotoxic T-lymphocyte precursors (CTLp) in chimeric mice.
    • Sendai-virus-specific CTLp were maintained for over 250 days in irradiated, uninfected recipients.
    • Virus-specific CD8+ memory T cells were maintained for over 100 days in naive recipients lacking detectable viral antibodies or protein depots.

    Conclusions:

    • CD8+ T-cell memory can be maintained for extended periods independent of continued viral antigen exposure.
    • The study found no evidence for persistent viral protein depots sustaining memory T-cell populations.
    • These findings challenge the necessity of persistent antigen for long-term virus-specific CD8+ T-cell memory.