[New studies on 3-dehydroecdysteroids in the biosynthetic pathway of ecdysone in Locusta migratoria]

Prothoracic glands of the locust, Locusta migratoria, incubated in vitro, converted in the same manner 3-dehydroketodiol (14 alpha-hydroxy-5 beta-cholest-7-en-3,6-dione) tritiated either on the side chain (22,23,24,25)3H4 or on the nucleus (1,2)3H2. Conversion products always appeared in two forms: one oxidized at C-3 corresponding with 3-dehydroecdysteroids, and the other corresponding with "classical" ecdysteroids which are usually obtained by conversion of ketodiol. All the different intermediate ecdysteroids between ketodiol and ecdysone are presents. A non-hemolymphatic reductase is conceivably responsible for the conversion of 3-dehydroecdysteroids at one or several places in the course of the biosynthetic pathway. Quantitatively the two forms (oxidized or hydroxylated at C-3) appeared in changeable ratios according to the different ecdysteroids but with a prevailing tendency to 1:1. The specificity of the conversion from nucleus-tritiated-dehydroketodiol depended on an enormous production of polar degradation products (more than 50% of total radioactivity). Consequently the quantities of 3-dehydro- and 3-hydroxy-ecdysteroids were lower than those which could be obtained after the conversion of side-chain-tritiated-3-dehydroketodiol. By means of an incubation with locust-larval-hemolymph, each 3-dehydroecdysteroid was totally (or at least in a great part: 3-dehydro-2-deoxyecdysone) converted into the corresponding reduced ecdysteroid. This fact confirms the reducing function of the hemolymph.(ABSTRACT TRUNCATED AT 250 WORDS)