Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human polymorphonuclear leukocytes lack detectable nitric oxide synthase activity

L Yan1, R W Vandivier, A F Suffredini

  • 1Critical Care Medicine Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892.

Journal of Immunology (Baltimore, Md. : 1950)
|August 15, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precise Measurement of the e^{+}e^{-}→π^{+}π^{-}J/ψ Cross Section at Center-of-Mass Energies from 3.77 to 4.60 GeV.

Physical review letters·2017
Same author

[A report of 2016 advances in American Association for the Study of Liver Diseases].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2017
Same author

[Influence of short-term intensive insulin therapy on plasma concentration of lipoprotein-associated phospholipase A(2) and secretory phospholipase A(2) in newly diagnosed type 2 diabetic patients].

Zhonghua nei ke za zhi·2017
Same author

Population Pharmacokinetics and Pharmacodynamics of Benralizumab in Healthy Volunteers and Patients With Asthma.

CPT: pharmacometrics & systems pharmacology·2017
Same author

Amplitude Analysis of the Decays η^{'}→π^{+}π^{-}π^{0} and η^{'}→π^{0}π^{0}π^{0}.

Physical review letters·2017
Same author

[Prevalence and associated factors of female urinary incontinence in Hebei province].

Zhonghua fu chan ke za zhi·2017
Same journal

Crystal structure and immune single-cell atlas provide insights into the functional divergence of type I IFNs in fish.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

An MR1-specific nanobody capable of blocking MR1T cell activation.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

TGF-β controls developmental fate and functional identity of thymic γδ T cells.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Human polymorphonuclear leukocytes (PMNs) do not exhibit nitric oxide synthase (NOS) activity. Studies comparing PMNs and human aortic endothelial cells (HAECs) found no inducible NOS activity in PMNs.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Nitric oxide (NO) is crucial for regulating polymorphonuclear leukocyte (PMN) functions.
  • The presence and activity of nitric oxide synthase (NOS) in human PMNs remain a subject of scientific debate.

Purpose of the Study:

  • To investigate and clarify the existence of nitric oxide synthase (NOS) activity in human polymorphonuclear leukocytes (PMNs).
  • To compare NOS activity in human PMNs with that of human aortic endothelial cells (HAECs).

Main Methods:

  • Quantified NOS activity by measuring the conversion of L-arginine to L-citrulline.
  • Utilized the NOS inhibitor N omega-amino-L-arginine (L-NAA) to assess specificity.
  • Assessed NOS induction in PMNs using various inflammatory stimuli (LPS, IL-1 beta, IFN-gamma, TNF-alpha).

Related Experiment Videos

  • Compared whole-cell and fractionated cell assays in PMNs and HAECs.
  • Measured nitrite and nitrate release from PMNs.

    • Main Results:

    • L-arginine to L-citrulline conversion, a marker of NOS activity, was significantly lower in human PMNs compared to HAECs.
    • NOS activity in PMNs was not inducible by inflammatory cytokines or LPS administration.
    • L-NAA did not inhibit L-arginine conversion in PMNs, and nitrite/nitrate release was minimal.
    • Calmodulin inhibition did not affect PMN L-arginine conversion.

    Conclusions:

    • Human polymorphonuclear leukocytes (PMNs) do not express significant nitric oxide synthase (NOS) activity.
    • These findings resolve controversy regarding NOS expression in human PMNs, differentiating them from endothelial cells.