[Recent developments in the diagnosis of parapoxviruses]

¹Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin, Tierärztliche Fakultät der Ludwig-Maximilians-Universität München.

Tierarztliche Praxis

|June 1, 1994

Abstract:

Neutralizing and non-neutralizing monoclonal antibodies against parapoxviruses (PPV) were generated by immunizing BALB/c-mice with gradient-purified PPV Orf D-1701 or purified envelopes. Epitope specificity studies identified three distinct epitopes localized in the virus envelope. These antigenic sites allowed a differentiation between orf and stomatitis papulosa viruses. For a rapid diagnosis of parapoxviruses transmission-electron microscopy, immunofluorescence- or immunoperoxidase-staining, antigen capture ELISA, and polymerase-chain-reaction were used.

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Abstract:

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