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Flow-cytometric lymphocyte phenotyping using automated SimulSET Software gating procedure

V Vukelić1, I Svoboda-Beusan

  • 1Institute of Immunology, Zagreb, Croatia.

Acta Medica Croatica : Casopis Hravatske Akademije Medicinskih Znanosti
|January 1, 1994
PubMed
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Peripheral blood lymphocyte phenotypes were analyzed in healthy young adults using flow cytometry. Results show sex-based differences in lymphocyte subpopulations, with key gating steps detailed for SimulSET software.

Area of Science:

  • Immunology
  • Flow Cytometry
  • Hematology

Background:

  • Understanding normal lymphocyte phenotypes is crucial for diagnosing immune disorders.
  • Reference ranges for lymphocyte subpopulations can vary based on demographics and methodology.
  • Dual fluorescence analysis provides detailed immune cell profiling.

Purpose of the Study:

  • To determine peripheral blood lymphocyte phenotypes in healthy young adults.
  • To identify potential sex-based differences in lymphocyte subpopulations.
  • To describe the gating strategy used in SimulSET software for lymphocyte analysis.

Main Methods:

  • Dual fluorescence flow cytometry was employed.
  • Fluorochrome-labeled monoclonal antibodies (mAbs) were used for cell surface marker detection.

Related Experiment Videos

  • A whole blood lysis technique and FACScan flow cytometer with SimulSET software were utilized.

    • Main Results:

    • Lymphocyte subpopulations (percentage and absolute counts) were quantified.
    • Significant differences in certain lymphocyte values were observed between sexes.
    • The gating procedure within SimulSET software was elucidated.

    Conclusions:

    • Established reference values for lymphocyte phenotypes in young healthy adults.
    • Highlighted the influence of sex on peripheral blood lymphocyte composition.
    • Provided a methodological description for lymphocyte subset analysis using SimulSET software.