Flow-cytometric lymphocyte phenotyping using automated SimulSET Software gating procedure

The peripheral blood lymphocyte phenotypes of 20 healthy young normal subjects between the ages 21 and 29 were determined by dual fluorescence analysis with fluorochrome--labeled monoclonal antibodies (mAbs). Samples were prepared by a whole blood lysis technique and analysed on a FACScan flow cytometer using SimulSET Software. Values for lymphocyte subpopulations (percentage and absolute count) are presented. Some values are different in relation to sex. The most important steps in SimulSET software gating procedure of lymphocytes are also described.