Jove
Visualize
Contact Us

Related Experiment Videos

Flow-cytometric lymphocyte phenotyping using automated SimulSET Software gating procedure

V Vukelić1, I Svoboda-Beusan

  • 1Institute of Immunology, Zagreb, Croatia.

Acta Medica Croatica : Casopis Hravatske Akademije Medicinskih Znanosti
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PMA and doxorubicin decrease viability, MTT activity and expression of CD10 marker on NALM-1 leukemic cells.

Immunopharmacology and immunotoxicology·2006
Same author

The relevance of multidrug resistance-associated P-glycoprotein expression in the treatment response of B-cell chronic lymphocytic leukemia.

Haematologica·2000
Same author

[Analysis of lymphocyte immunophenotypes in neonates].

Lijecnicki vjesnik·2000
Same author

[Multiple drug resistance].

Lijecnicki vjesnik·1994
Same author

Multiple changes of immunologic parameters in prisoners of war. Assessments after release from a camp in Manjaca, Bosnia.

JAMA·1993
Same author

T-cell subsets in asthmatic children.

Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti·1993
Same journal

[CHARACTERISTIC FEATURES OF PRESSURE ULCER INFECTION].

Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti·2017
Same journal

[PERSONALIZED APPROACH TO PATIENT WITH CHRONIC WOUND IN FAMILY MEDICINE].

Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti·2017
Same journal

[APPLICATION OF TOPICAL HEMOGLOBIN SPRAY IN INFECTED POSTTRAUMATIC AND POSTOPERATIVE WOUNDS].

Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti·2017
Same journal

[APPLICATION OF NEGATIVE PRESSURE THERAPY].

Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti·2017
Same journal

[PATIENTS WITH PRESSURE ULCERS IN THE UNIFIED EMERGENCY ADMISSION DEPARTMENT – OUR REALITY].

Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti·2017
Same journal

[Proposition of algorhythm for treatment with hyperbaric oxygenation Hyperbaric oxygenation in Rijeka Clinical Hospital Center].

Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti·2017
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Peripheral blood lymphocyte phenotypes were analyzed in healthy young adults using flow cytometry. Results show sex-based differences in lymphocyte subpopulations, with key gating steps detailed for SimulSET software.

Area of Science:

  • Immunology
  • Flow Cytometry
  • Hematology

Background:

  • Understanding normal lymphocyte phenotypes is crucial for diagnosing immune disorders.
  • Reference ranges for lymphocyte subpopulations can vary based on demographics and methodology.
  • Dual fluorescence analysis provides detailed immune cell profiling.

Purpose of the Study:

  • To determine peripheral blood lymphocyte phenotypes in healthy young adults.
  • To identify potential sex-based differences in lymphocyte subpopulations.
  • To describe the gating strategy used in SimulSET software for lymphocyte analysis.

Main Methods:

  • Dual fluorescence flow cytometry was employed.
  • Fluorochrome-labeled monoclonal antibodies (mAbs) were used for cell surface marker detection.

Related Experiment Videos

  • A whole blood lysis technique and FACScan flow cytometer with SimulSET software were utilized.

    • Main Results:

    • Lymphocyte subpopulations (percentage and absolute counts) were quantified.
    • Significant differences in certain lymphocyte values were observed between sexes.
    • The gating procedure within SimulSET software was elucidated.

    Conclusions:

    • Established reference values for lymphocyte phenotypes in young healthy adults.
    • Highlighted the influence of sex on peripheral blood lymphocyte composition.
    • Provided a methodological description for lymphocyte subset analysis using SimulSET software.