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Autoantibodies against a novel DNA-binding protein: DNA-protein interaction as a requisite for expression of
1Department of Immunology, Hospital de Sant Pau, Universitat Autonoma de Barcelona, Spain.
Clinical Immunology and Immunopathology
|August 1, 1994
Abstract:
A novel DNA-binding protein complex, the HB complex, has been characterized by means of autoantibodies. Three proteins of 9000, 7500, and 7000 Da constitute the HB complex. The 9000- and 7500-Da proteins are phosphorylated. Autoantibodies recognize the 7000-Da protein when it is bound to DNA. No reactivity against any protein was observed when the complex was dissociated from DNA. The three proteins are acidic (pI 5-6.2), and the complex was able to bind to synthetic double-stranded DNAs of different composition.