Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutation induction by accelerated heavy ions in bacteria

S Kozubek1, L Rýznar, G Horneck

  • 1Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno.

Mutation Research
|August 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Letter to the Editor.

Radiation protection dosimetry·2016
Same author

Ellagic Acid-Changed Epigenome of Ribosomal Genes and Condensed RPA194-Positive Regions of Nucleoli in Tumour Cells.

Folia biologica·2015
Same author

Overview of the Liulin type instruments for space radiation measurement and their scientific results.

Life sciences in space research·2015
Same author

CARM1 modulators affect epigenome of stem cells and change morphology of nucleoli.

Physiological research·2015
Same author

Frequent chromatin rearrangements in myelodysplastic syndromes--what stands behind?

Folia biologica·2014
Same author

Advanced microscopy techniques used for comparison of UVA- and γ-irradiation-induced DNA damage in the cell nucleus and nucleolus.

Folia biologica·2014

Heavy ion irradiation induces mutations in bacteria, with mutation rates depending on particle energy and linear energy transfer (LET). Mutation cross-sections vary significantly across different LET ranges, indicating complex biological responses to radiation.

Area of Science:

  • Radiation biology
  • Molecular genetics
  • Astrobiology

Background:

  • Heavy ions are a significant component of galactic cosmic radiation.
  • Understanding their mutagenic potential is crucial for assessing risks in space exploration and on Earth.
  • Bacterial mutation assays provide sensitive models for studying DNA damage and repair mechanisms.

Purpose of the Study:

  • To investigate the mutagenic effects of heavy ions across a range of atomic numbers (Z = 1-36) and energies (1-600 MeV/u).
  • To determine the relationship between mutation induction, particle energy, and linear energy transfer (LET).
  • To identify distinct response patterns in mutation induction based on LET values.

Main Methods:

  • Utilized Escherichia coli Ymcl and Salmonella typhimurium TA102 as model organisms.

Related Experiment Videos

  • Irradiated bacteria with heavy ions (Z = 1-36) at specific energies (1-600 MeV/u).
  • Quantified mutation induction using lacI forward mutation assays and his(-)-->his+ reversion assays.
  • Analyzed mutation induction cross-section (sigma m) as a function of particle energy and LETinfinity.

    • Main Results:

    • Observed a strong dependence of mutation induction cross-section (sigma m) on both particle energy and LETinfinity.
    • Identified two distinct ranges of LETinfinity with different mutagenic responses.
    • In high LETinfinity (> 100 keV/micron), sigma m increased with particle energy (e.g., Fe ions vs. C or alpha-particles).
    • In low LETinfinity (< 100 keV/micron), sigma m decreased with increasing energy (e.g., Ne ions vs. He ions).

    Conclusions:

    • Bacterial mutation induction by heavy ions is complex and highly dependent on energy and LET.
    • The observed differential responses suggest distinct mechanisms of DNA damage and repair are activated at different LET values.
    • These findings have implications for radiation protection strategies in space and for understanding biological effects of high-energy particles.