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CD14 is expressed and functional in human B cells

H W Ziegler-Heitbrock¹, H Pechumer, I Petersmann

¹Institute for Immunology, University of Munich, Germany.

European Journal of Immunology

|August 1, 1994

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Human B cells express functional CD14, a molecule involved in immune responses. This study identified CD14 on B cells and demonstrated its role in lipopolysaccharide-induced cytokine production, offering new insights into B cell immunology.

Area of Science:

Immunology
Cell Biology

Background:

The human B cell line RPMI 8226 shows inconsistent CD14 antibody staining.
CD14 is a well-characterized monocyte marker.

Purpose of the Study:

To investigate the expression and function of CD14 in human B cells.
To determine if CD14 on B cells is functional and mediates responses to lipopolysaccharide (LPS).

Main Methods:

Isolation and characterization of CD14-expressing B cell clones from RPMI 8226.
Immunoprecipitation, Northern blotting, and cytokine assays (IL-6, IL-10) to assess CD14 expression and function.
Functional blocking assays using anti-CD14 antibody and antiserum.
Purification of primary human B cells via cell sorting.

Main Results:

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Two stable B cell clones (1 and 2) expressed CD14, while clone 3 did not.
CD14 expression was associated with LPS-induced production of IL-6 and IL-10.
Anti-CD14 antibody blocked LPS-induced cytokine production in B cell clones.
Primary human B cells also expressed functional CD14, responding to LPS with IL-6 production that was blocked by anti-CD14 antiserum.

Conclusions:

Human B cells express functionally active CD14.
CD14 on B cells plays a role in mediating inflammatory responses to lipopolysaccharide.
These findings expand the known roles of CD14 beyond monocytes.