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Aggrecan in bovine tendon

K G Vogel1, J D Sandy, G Pogány

  • 1Department of Biology, University of New Mexico, Albuquerque.

Matrix Biology : Journal of the International Society for Matrix Biology
|March 1, 1994
PubMed
Summary
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Aggrecan, a large proteoglycan, is the main component in compressed bovine tendon regions, similar to cartilage. Tensional tendon regions contain aggrecan fragments lacking the G1 domain.

Area of Science:

  • Biochemistry
  • Biomaterials Science
  • Molecular Biology

Background:

  • Large proteoglycans are crucial components of connective tissues, influencing their mechanical properties.
  • Aggrecan is the predominant proteoglycan in articular cartilage, providing hydration and load-bearing capacity.

Purpose of the Study:

  • To investigate the composition and structure of large proteoglycans in different regions of adult bovine deep flexor tendon.
  • To compare tendon aggrecan with that found in articular cartilage.

Main Methods:

  • Proteoglycan purification using ion-exchange chromatography, gel filtration, and CsCl gradient centrifugation.
  • Tryptic peptide mapping and sequencing to identify protein domains.
  • Northern blot analysis to assess aggrecan mRNA expression.

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Main Results:

  • Aggrecan, structurally similar to cartilage aggrecan, is the predominant proteoglycan in compressed tendon regions.
  • Tensional tendon regions contain aggrecan, but with a notable absence of G1 domain peptides.
  • Aggrecan mRNA is highly expressed in compressed tendon and cartilage cells, with lower levels in tensional tendon.

Conclusions:

  • Aggrecan is the primary chondroitin sulfate proteoglycan in compressed bovine tendon.
  • The presence of aggrecan fragments lacking the G1 domain explains the proteoglycans found in tensional tendon regions.