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In vitro fertilization in couples with previous fertilization failure using sperm incubated with pentoxifylline and
H Tournaye1, R Janssens, G Verheyen
1Centre for Reproductive Medicine, University Hospital, Brussels Free University, Belgium.
Objective:
To evaluate whether incubation of spermatozoa with both pentoxifylline and 2-deoxyadenosine would improve fertilization rates in couples with previous IVF failure.
Design:
Autocontrolled design in which sibling oocytes were inseminated at random in vitro with spermatozoa treated or not treated by pentoxifylline and 2-deoxyadenosine.
Mean Outcome Measures:
Oocyte quality, sperm motility, fertilization in vitro, and embryo quality.
Results:
Sperm motility was found optimized by metabolic stimulation using pentoxifylline and 2-deoxyadenosine. The mean fertilization rate per patient was 33.1% in the treatment group compared with 37.0% in the control group. The mean cleavage rate per patient was 79.6% for treatment versus 68.7% for control embryos. No differences in embryo quality were noted.
Conclusion:
The results of this study demonstrate that an indiscriminate use of pentoxifylline and 2-deoxyadenosine is not beneficial to fertilization in couples with previous IVF failure. Further prospective research may be needed to assess the benefit of pentoxifylline and 2-deoxyadenosine in patients selected by preliminary functional in vitro tests.