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Related Experiment Videos

Altered angiogenesis underlying age-dependent changes in tumor growth

R Pili1, Y Guo, J Chang

  • 1Cell Biology Unit, National Institute of Aging, National Institutes of Health, Baltimore, Md 21224.

Journal of the National Cancer Institute
|September 7, 1994
PubMed
Summary

Tumor growth and vascularization are impaired in older mice due to reduced angiogenic factors and increased extracellular matrix. Targeting tumor vascularization may offer a strategy to reduce tumor growth and progression in elderly patients.

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Area of Science:

  • Oncology
  • Aging Research
  • Vascular Biology

Background:

  • Tumor growth and spread slow in the elderly, with murine models showing reduced tumor growth and spread in older animals.
  • Neovascularization is critical for tumor growth, and age-related alterations in vascular response may impact tumor progression.

Purpose of the Study:

  • To investigate age-related differences in tumor growth and vascularization using transplantable tumor cells, tumors, and extracts.
  • To understand how host age influences tumor biology and angiogenesis.

Main Methods:

  • Englebreth-Holm-Swarm (EHS) carcinoma and B16-F10 melanoma cells were implanted in C57BL mice of varying ages.
  • Tumor growth, histology, DNA synthesis, and vascularization (using basic fibroblast growth factor and in vivo angiogenesis assay) were assessed.

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  • Tumor extracts were tested for their effects on endothelial cell differentiation in vitro and tumor growth in vivo.

    • Main Results:

    • EHS tumors grew larger in young mice than in old mice, with rapid growth resuming upon transfer to young hosts.
    • Tumors from old mice showed a higher extracellular matrix to tumor cell ratio and fewer, larger blood vessels compared to tumors from young mice.
    • Young mice exhibited a stronger angiogenic response; tumor extracts from old mice impaired endothelial cell differentiation and reduced tumor growth in vivo.

    Conclusions:

    • Tumor growth and morphology are age-dependent, influenced by reduced vascularization capacity due to diminished angiogenic factors or presence of host inhibitors.
    • Age-related changes in host factors may affect tumor growth and tissue repair, suggesting that reducing tumor vascularization is a potential therapeutic target.