Detection of immunoreactive substance P-like substances from cat brainstem sites during fatiguing isometric...

Isometric contractions were generated on the left hindlimb muscles from adult cats (n = 11) anesthetized with alpha-chloralose (75 mg/kg) to determine whether immunoreactive substance P (irSP) was released from either the right periaqueductal grey (PAG) or ventrolateral medulla (VLM), sites shown to be involved with the integration of the muscle pressor response. The release of immunoreactive SP was measured using SP antibody-coated microelectrodes that were inserted into the PAG or the VLM during periods of rest, fatiguing isometric contractions and post-contraction. Mean arterial pressure increased by 78 +/- 11 mmHg during the contractions. There was a release of irSP from sites in the medulla during the contractions compared to the non-contraction periods but none was detected from the PAG in response to muscle stimulation. These results provide further evidence that SP-like substances may be involved with the central integration of the muscle pressor response.