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Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro
S De Angeli1, A Fandella, M T Conconi
1Laboratory of Cellular Cultures, Regional Hospital USSL, Treviso, Italy.
Abstract:
This work studies the effects of suramin on the growth and morphology of cell strain U285, obtained from human prostate hypertrophic tissue and cultured in vitro. The FRAME cytotoxicity test was performed to evaluate the inhibition of growth induced by suramin. Cells were exposed to suramin at the time of seeding and 24 hours later; neutral red was added with and without suramin. An optical microscope connected to a computer-aided system and a scanning electron microscope were used to study morphological changes induced by suramin. Growth inhibition depends on drug concentration and exposure period. Moreover, the effect of suramin on neutral red uptake is reversible. Suramin 1,000 microM causes the cells to become spheroid, and they fail to form a monolayer. Our data indicate that the addition of suramin during the lag phase decreases the rate of cell proliferation.
Insights
Suramin inhibits prostate cell growth and alters cell shape, with effects dependent on concentration and exposure time. This drug impacts cell proliferation, particularly when introduced during the lag phase of cell growth.
Area of Science:
- Cell Biology
- Pharmacology
- Prostate Cancer Research
Background:
- Human prostate hypertrophic tissue cell strain U285 cultured in vitro.
- Suramin's known biological activities.
Purpose of the Study:
- Investigate suramin's effects on U285 cell growth and morphology.
- Evaluate suramin's cytotoxicity using the FRAME assay.
- Analyze morphological changes induced by suramin exposure.
Main Methods:
- In vitro cell culture of U285 cells.
- FRAME cytotoxicity test with neutral red uptake.
- Microscopic analysis (optical and scanning electron microscopy).
Main Results:
- Suramin inhibits cell growth in a dose- and time-dependent manner.
- Morphological changes include spheroid formation and failure to form a monolayer at 1,000 microM.
- Suramin's effect on neutral red uptake is reversible.
- Suramin addition during the lag phase reduces cell proliferation rate.
Conclusions:
- Suramin exhibits cytotoxic and morphological effects on U285 prostate cells.
- The drug's impact on cell proliferation is significant, especially during early growth phases.
- Suramin demonstrates potential as an agent affecting prostate cell behavior in vitro.