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Pseudomonas flavescens sp. nov., isolated from walnut blight cankers
D C Hildebrand1, N J Palleroni, M Hendson
1Department of Plant Pathology, University of California, Berkeley 94720.
International Journal of Systematic Bacteriology
|July 1, 1994
Summary
Two new pseudomonad strains isolated from walnut trees were identified as a new species, Pseudomonas flavescens. These bacteria produce yellow and fluorescent pigments, aiding in their classification.
Area of Science:
- Microbiology
- Plant Pathology
- Bacteriology
Background:
- Pseudomonad strains were isolated from walnut tree cankers.
- These isolates produced yellow cellular and diffusible fluorescent pigments.
Purpose of the Study:
- To characterize and classify two novel pseudomonad strains.
- To determine if these strains represent a new species within the Pseudomonas genus.
Main Methods:
- Biochemical tests (oxidase, arginine dihydrolase).
- Nutritional characterization.
- DNA-DNA hybridization.
- PCR-amplified 16S rRNA gene sequencing.
- Genomic DNA base composition analysis (G+C content).
Main Results:
- The strains exhibited specific biochemical profiles and pigment production.
- DNA-DNA hybridization and 16S rRNA gene sequencing supported classification within Pseudomonas rRNA group I.
- Genomic DNA base composition was 63 mol% G+C.
- The strains were distinct from known Pseudomonas species.
Conclusions:
- The two isolated pseudomonad strains represent a new species.
- The proposed name for this new species is Pseudomonas flavescens.
- Strain B62 is designated as the type strain for Pseudomonas flavescens.