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Updated: Aug 19, 2026
Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos
Published on: June 12, 2020
Improvement in blastocyst hatching of mouse embryos cocultured with human placental cells
T Morishita1, M Yokoyama, M Nozaki
1Department of Gynecology and Obstetrics, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Purpose:
Although the hatching of embryos is an important phenomenon, the mechanism of hatching remains controversial. Therefore, we attempted to develop a new coculture system with human placental cells to investigate the hatching of mouse embryos.
Results:
In our new system there was no difference in development from the two-cell stage to blastocysts between embryos cultured with a T6 medium and embryos cocultured with human placental cells at 1 x 10(5), 5 x 10(5), and 1 x 10(6) cells/ml. However, the hatching-rate cell number increased significantly in embryos cocultured with placental cells compared to embryos cultured without placental cells. [3H]Thymidine uptake did not show any significant difference from the beginning of in vitro culture to the hatching stage between the coculture group and the control group. Nevertheless, the [3H]uridine uptake was significantly different in the two groups, measuring 2167 +/- 532 cpm/10 embryos in the coculture group and 804 +/- 86 cpm/10 embryos in the control group at 114 hr after human chorionic gonadotropin injection (P < 0.01).
Conclusion:
These results therefore seem to indicate that the hatching of blastocysts depends on the protein synthesis of the embryos and not on DNA duplications.
