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Quantitative analysis of soluble cell adhesion molecules in otitis media with effusion
T Himi1, M Kamimura, A Kataura
1Department of Otolaryngology, Sapporo Medical University, Japan.
Abstract:
The levels of the soluble intercellular adhesion molecule 1 (sICAM-1) and soluble granule membrane protein-140 (sGMP-140) were measured in middle ear effusions (MEEs) of otitis media (OM) with ELISA. MEEs of chronic serous and mucoid OM in children contained significantly higher levels of sICAM-1 and sGMP-140 compared with normal serum. sGMP-140 in acute and chronic OM in children was higher than in adult chronic serous OM, and in acute purulent OM higher chronic serous OM in children. Cytologic analysis showed that the mean level of sGMP-140 was significantly higher in the neutrophil dominant group than in other groups except the few cells type. sICAM-1 showed no differences among the cytologic classifications. Copious amounts of sICAM-1 and sGMP-140 in MEEs were observed in this study, and were probably the result of shedding from the cells expressed cell adhesion molecules. These cell adhesion molecules in MEEs were thought to modify the inflammatory response in OM.
Insights
Researchers measured soluble intercellular adhesion molecule 1 (sICAM-1) and soluble granule membrane protein-140 (sGMP-140) in middle ear effusions (MEEs) from otitis media (OM) patients. Elevated levels of sICAM-1 and sGMP-140 were found in children with OM, suggesting their role in the inflammatory response.
Area of Science:
- Biochemistry
- Immunology
- Otolaryngology
Background:
- Otitis media (OM) is a common middle ear inflammation.
- Cell adhesion molecules (CAMs) play a role in inflammatory processes.
- Soluble forms of CAMs, such as sICAM-1 and sGMP-140, may indicate inflammatory activity.
Purpose of the Study:
- To quantify levels of sICAM-1 and sGMP-140 in middle ear effusions (MEEs) of patients with otitis media.
- To compare these levels between different types of OM and age groups.
- To investigate the relationship between sICAM-1, sGMP-140, and cytologic findings in MEEs.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to measure sICAM-1 and sGMP-140 levels in MEEs.
- MEEs were collected from children and adults with various forms of OM (acute, chronic, serous, mucoid, purulent).
- Cytologic analysis of MEEs was performed to assess cell populations.
Main Results:
- MEEs from children with chronic serous and mucoid OM showed significantly higher sICAM-1 and sGMP-140 levels compared to normal serum.
- sGMP-140 levels were higher in acute and chronic OM in children compared to adult chronic serous OM.
- Acute purulent OM in children exhibited higher sGMP-140 levels than chronic serous OM in children.
- sGMP-140 levels were significantly elevated in neutrophil-dominant MEEs.
- sICAM-1 levels did not differ across cytologic classifications.
Conclusions:
- Elevated sICAM-1 and sGMP-140 are present in MEEs of otitis media, particularly in children.
- These soluble adhesion molecules likely originate from shedding cells and may modulate the inflammatory response in OM.
- sGMP-140 levels correlate with neutrophil presence, indicating its role in acute inflammation.