Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selective interference with P protein binding to paramyxovirus nucleocapsids

K W Ryan1, U F Power

  • 1Department of Virology & Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38101.

Virus Research
|June 1, 1994
PubMed
Summary

Monoclonal antibodies can displace P protein from Sendai virus nucleocapsids and related human parainfluenza virus type 1 (PIV1) nucleocapsids. A single antibody blocks P protein binding to a large region of the nucleocapsid core.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibody-Induced Internalization of the Human Respiratory Syncytial Virus Fusion Protein.

Journal of virology·2017
Same author

Report of the 2nd "French Clinical Vaccinology Meeting Jean-Gerard Guillet": immunization and respiratory diseases.

Vaccine·2010
Same author

Structure-antigenicity relationship studies of the central conserved region of human respiratory syncytial virus protein G.

The journal of peptide research : official journal of the American Peptide Society·2002
Same author

Safety and immunogenicity of a novel recombinant subunit respiratory syncytial virus vaccine (BBG2Na) in healthy young adults.

The Journal of infectious diseases·2001
Same author

Differential histopathology and chemokine gene expression in lung tissues following respiratory syncytial virus (RSV) challenge of formalin-inactivated RSV- or BBG2Na-immunized mice.

Journal of virology·2001
Same author

A novel bipolar mode of attachment to aluminium-containing adjuvants by BBG2Na, a recombinant subunit hRSV vaccine.

Vaccine·2001

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Sendai virus (SV) P protein interacts with nucleocapsid cores.
  • Anti-nucleoprotein (NP) monoclonal antibodies can interfere with SV P protein binding.

Purpose of the Study:

  • To investigate if anti-NP antibody-mediated P protein displacement occurs with other paramyxoviruses.
  • To determine the stoichiometry of antibody interference with P protein binding to nucleocapsids.

Main Methods:

  • Used anti-NP antibodies to test binding interference with Sendai virus (SV) and human parainfluenza virus type 1 (PIV1) nucleocapsids.
  • Assessed the effect of antibody binding on P protein attachment to viral nucleocapsids.
  • Quantified the number of antibody molecules required to block P protein binding.

Related Experiment Videos

Main Results:

  • Anti-NP antibodies displaced P protein from both SV and PIV1 nucleocapsids.
  • Antibodies blocked SV P protein binding to SV and PIV1 nucleocapsids.
  • Antibodies blocked PIV1 P protein binding to PIV1 nucleocapsids but not SV nucleocapsids.
  • Approximately 40 antibody molecules per nucleocapsid blocked most P protein attachment.

Conclusions:

  • Antibody-mediated P protein displacement from nucleocapsids is not unique to SV.
  • A single antibody molecule can prevent P protein binding to a significant region (approx. 65 NP monomers) of the nucleocapsid core.